Anti-Adenylate Cyclase 3 Antibody (A104341)

Immunofluorescence analysis of rat cortex section stained with Anti-ACIII Antibody (1:10,000 | red) and co-stained with Anti-CNP Antibody (1:1,000 | green). The blue is Hoechst staining of nuclear DNA. The Anti-ACIII Antibody reveals neuronal cilia while the Anti-CNP Antibody stains oligodendrocytes and the myelin sheath around axons.

Western blot analysis of different tissue lysates using Anti-ACIII Antibody (1:1,000 | green). On the left blot: [1] protein standard, [2] rat hippocampus, [3] mouse hippocampus, [4] mouse frontal cortex, and [5] cow frontal cortex. Anti-ACIII Antibody detects variably glycosylated forms of ACIII protein with apparent molecular weights from ~120kDa and higher. On the right blot: [6] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with DNA expressing Myc-DDK tagged full length human adenylate cyclase III from the appropriate cDNA (ACIII). The strong band at about 130kDa demonstrates overexpression of the ACIII protein, and those over 250kDA double band presumably corresponds to heavily glycosolated or aggregated forms of ACIII. The same blot was simultaneously probed with Anti-GAPDH Antibody (1:5,000 | red), which reveals the single GAPDH band at ~37kDa in both transfected and non-transfected cells.