Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
A disintegrin and metalloproteinase with thrombospondin motifs 11, A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAM-TS 11, ADAM-TS 5, ADAM-TS5, ADAMTS-11, ADAMTS-5, ADAMTS11, ADMP-2, ADMP2, Aggrecanase-2
Western blot analysis of extracts of various cell lines, using Anti-ADAMTS5 Antibody (A14217) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-ADAMTS5 Antibody (A14217) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U-87 MG cells using Anti-ADAMTS5 Antibody (A14217) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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