Anti-ADAM17 Antibody (A16782)

Name: Anti-ADAM17 Antibody
Brand: Antibodies.com
SKU: A16782
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ADAM17 for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Highlighted products for Anti-ADAM17 Antibody

A83218

Anti-ADAM17 Antibody

Goat polyclonal antibody to ADAM17 for ELISA and WB.

A83218

Anti-ADAM17 Antibody

Goat polyclonal antibody to ADAM17 for ELISA and WB.

Specifications

Name

Anti-ADAM17 Antibody

Description

Rabbit polyclonal antibody to ADAM17.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human ADAM17 (NP_003174.3).

Sequence

KQYESLSLFHPSNVEMLSSMDSASVRIIKPFPAPQTPGRLQPAPVIPSAPAAPKLDHQRMDTIQEDPSTDSHMDEDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQNRVDSKETEC

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 kDa / 100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

A disintegrin and metalloproteinase domain 17, A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), ADA17_HUMAN, ADAM 17, ADAM metallopeptidase domain 17, ADAM17 protein, CD 156b, CD156b, CD156b antigen, CSVP, Disintegrin and metalloproteinase domain-containing protein 17, MGC71942, NISBD, NISBD1, Snake venom like protease, Snake venom-like protease, TACE, TNF alpha convertase, TNF alpha converting enzyme, TNF-alpha convertase, TNF-alpha-converting enzyme, Tumor Necrosis Factor Alpha Converting Enzyme

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ADAM17 Antibody (A16782)

Western blot analysis of extracts of various cell lines, using Anti-ADAM17 Antibody (A16782) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-ADAM17 Antibody (A16782)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-ADAM17 Antibody (A16782) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-ADAM17 Antibody (A16782)

Immunofluorescence analysis of HeLa cells using Anti-ADAM17 Antibody (A16782) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-ADAM17 Antibody (A16782)

Immunofluorescence analysis of NIH/3T3 cells using Anti-ADAM17 Antibody (A16782) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).