Anti-ADAM15 Antibody [ARC1419] (A308455)

Name: Anti-ADAM15 Antibody [ARC1419]
Brand: Antibodies.com
SKU: A308455
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1419] antibody to ADAM15 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ADAM15 Antibody [ARC1419]

Description

Rabbit monoclonal [ARC1419] antibody to ADAM15.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ADAM15 (Q13444).

Sequence

RHIRRRRDVVTETKTVELVIVADHSEAQKYRDFQHLLNRTLEVALLLDTFFRPLNVRVALVGLEAWTQRDLVEISPNPAVTLENFLHWRRAHLLPRLPHDS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1419

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

A disintegrin and metalloproteinase domain 15, A disintegrin and metalloproteinase domain 15 (metargidin), ADA15_HUMAN, ADAM 15, ADAM metallopeptidase domain 15, and cysteine-rich protein 15, Disintegrin and metalloproteinase domain-containing protein 15, disintegrin-like, EC 3.4.24., MDC 15, MDC-15, MDC15, Metalloprotease RGD disintegrin protein, Metalloproteinase like disintegrin like and cysteine rich protein 15, Metalloproteinase-like, Metargidin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-ADAM15 Antibody [ARC1419] (A308455)

Western blot analysis of extracts of HCT116 cells, using Anti-ADAM15 Antibody [ARC1419] (A308455) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-ADAM15 Antibody [ARC1419] (A308455)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-ADAM15 Antibody [ARC1419] (A308455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ADAM15 Antibody [ARC1419] (A308455)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-ADAM15 Antibody [ARC1419] (A308455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-ADAM15 Antibody [ARC1419] (A308455)

Immunofluorescence analysis of rat rectum using Anti-ADAM15 Antibody [ARC1419] (A308455) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).