Anti-ACTN3 Antibody (A87751)

Name: Anti-ACTN3 Antibody
Brand: Antibodies.com
SKU: A87751
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ACTN3 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ACTN3 Antibody

Description

Rabbit polyclonal antibody to ACTN3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 580-630 of human ACTN3 (NP_001095.2).

Sequence

GAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSCDQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

103 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Actinin alpha 3, actinin, alpha, ACTN 3, ACTN3_HUMAN, Alpha actinin skeletal muscle, Alpha actinin skeletal muscle isoform 3, Alpha-Actinin 3, Alpha-actinin skeletal muscle isoform 3, Alpha-actinin-3, F actin cross linking protein, F-actin cross-linking protein, MGC117002, MGC117005, OTTHUMP00000219049, OTTHUMP00000219054

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-ACTN3 Antibody (A87751)

Western blot analysis of extracts of various cell lines, using Anti-ACTN3 Antibody (A87751) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-ACTN3 Antibody (A87751)

Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using Anti-ACTN3 Antibody (A87751) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ACTN3 Antibody (A87751)

Immunohistochemistry analysis of paraffin-embedded rat skeletal muscle using Anti-ACTN3 Antibody (A87751) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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