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Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644)

Western Blot - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) - Antibodies.com Western Blot - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) - Antibodies.com Western Blot - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) - Antibodies.com Immunohistochemistry - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) - Antibodies.com Immunohistochemistry - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) - Antibodies.com
Knockout Validated
$470
Product Datasheet

Rabbit polyclonal antibody to Actin Regulatory Protein CAPG / MCP for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Actin Regulatory Protein CAPG / MCP Antibody
Description
Rabbit polyclonal antibody to Actin Regulatory Protein CAPG / MCP.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2).
Sequence
MYTAIPQSGSPFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGGVESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGGKSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFISRMQYAPNTQVEILPQGHESPIFKQFFKDWK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
38 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644)
Western blot analysis of extracts of various cell lines, using Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644)
Western blot analysis of extracts of various cell lines, using Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644)
Western blot analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Actin Regulatory Protein CAPG / MCP Antibody (A15644) at a dilution of 1:100 (40x lens).
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