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Anti-ACTC1 Antibody (A80440)

Western Blot - Anti-ACTC1 Antibody (A80440) - Antibodies.com Immunohistochemistry - Anti-ACTC1 Antibody (A80440) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to ACTC1 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-ACTC1 Antibody
Description
Rabbit polyclonal antibody to ACTC1.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ACTC1 (NP_005150.1).
Sequence
MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ACTC, ACTC_HUMAN, Actin, Actin alpha cardiac muscle 1, alpha cardiac muscle 1, Alpha-cardiac actin, ASD5, CMD1R, CMH11, LVNC4
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ACTC1 Antibody (A80440) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ACTC1 Antibody (A80440)
Western blot analysis of extracts of various cell lines, using Anti-ACTC1 Antibody (A80440) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-ACTC1 Antibody (A80440) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-ACTC1 Antibody (A80440)
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle using Anti-ACTC1 Antibody (A80440) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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