Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201] (A308476)

Name: Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201]
Brand: Antibodies.com
SKU: A308476
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2201] antibody to Acetyl Coenzyme A carboxylase alpha for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

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Specifications

Name

Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201]

Description

Rabbit monoclonal [ARC2201] antibody to Acetyl Coenzyme A carboxylase alpha.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human ACC1 (Q13085).

Sequence

RLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2201

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

277 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ACAC, ACACA, ACACA_HUMAN, ACC alpha, ACC-alpha, ACC1, ACCA, Acetyl Coenzyme A, Biotin carboxylase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201] (A308476)

Western blot analysis of extracts of various cell lines, using Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201] (A308476) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201] (A308476)

Western blot analysis of extracts of Mouse heart, using Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201] (A308476) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201] (A308476)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201] (A308476) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201] (A308476)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Acetyl Coenzyme A carboxylase alpha Antibody [ARC2201] (A308476) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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