Principle of Assay
This kit provides a simple, sensitive, rapid colorimetric acetyl-CoA detection method. Suitable for various types of samples, especially animals or plant tissues or cells. The detection principle is that malate dehydrogenase can catalyze the reaction of malate acid and NAD+ to produce oxaloacetic acid and NADH. Citrate synthase catalyzes the reaction of acetyl-CoA and oxaloacetic acid to produce citric acid and CoA. Based on the coupling reaction of malate dehydrogenase and citrate synthase, acetyl-CoA content is proportional to the generation of NADH. NADH has an absorption peak at 340 nm, and the measurement of the absorbance value of 340 nm enables calculation of the content of acetyl-CoA.
