Anti-ABCC9 Antibody [S319A-14] (A304816)

Name: Anti-ABCC9 Antibody [S319A-14]
Brand: Antibodies.com
SKU: A304816
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S319A-14] antibody to ABCC9 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ABCC9 Antibody [S319A-14]

Description

Mouse monoclonal [S319A-14] antibody to ABCC9.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with SUR2B.

Immunogen

Fusion protein amino acids 1505-1546 (SSIVDAGLVLVFSEGILVECDTGPNLLQHKNGLFSTLVMTNK, cytoplasmic C-terminus) of mouse SUR2A.

Host

Mouse

Clonality

Monoclonal

Clone ID

S319A-14

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ABC37, ABCC9_HUMAN, AI414027, AI449286, ATFB12, ATP-binding cassette sub-family C member 9, ATP-binding cassette transporter sub-family C member 9, ATP-binding cassette, sub-family C (CFTR/MRP), member 9, CANTU, CMD1O, FLJ36852, Sulfonylurea receptor 2, Sulfonylurea-binding protein 2, SUR2, SUR2A, SUR2B

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-ABCC9 Antibody [S319A-14] (A304816)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-ABCC9 Antibody [S319A-14] (A304816), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR2A Antibody (D) Composite.

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Figure 2: Western Blot - Anti-ABCC9 Antibody [S319A-14] (A304816)

Western blot analysis of rat brain membrane showing detection of ~120 kDa SUR2A protein using Anti-ABCC9 Antibody [S319A-14] (A304816) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat brain membrane (10 µg).. Load: 10 µg. Block: 5% milk. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at room temperature. Color Development: TMB solution for 10 min at room temperature. Predicted/Observed Size: ~120 kDa.

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Figure 3: Immunofluorescence - Anti-ABCC9 Antibody [S319A-14] (A304816)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ABCC9 Antibody [S319A-14] (A304816), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SUR2A Antibody. (D) Composite.

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