Recombinant Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free (A323871)

Name: Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free
Brand: Antibodies.com
SKU: A323871
Price: 295.0 USD
Availability: InStock

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$295

Recombinant human monoclonal [PF-06263507] antibody to 5T4 for ELISA, FACS, Functional Assay and In Vivo.

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7-10 Business Days

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Recombinant Antibody

Specifications

Name

Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free

Description

Recombinant human monoclonal [PF-06263507] antibody to 5T4.

Applications

ELISA, FACS, Functional Assay, In Vivo

Reactivity

Human, Cynomolgus Macaque

Host

Human

Clonality

Monoclonal

Clone ID

PF-06263507

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein A affinity chromatography.

Concentration

Reconstitution dependent.

Molecular Weight

This antibody has a predicted MW of 145.8 kDa.

Purity

> 95% (by SDS-PAGE and SEC-HPLC).

Product Form

Lyophilized

Reconstitution

Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!

Formulation

Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, 5T4 oncotrophoblast glycoprotein, 5T4AG, AW495680, M6P1, TPBG, TPBG_HUMAN, Trophoblast glycoprotein

Suitable Secondaries

See all Anti-Human IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: SDS-PAGE - Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free (A323871)

SDS-PAGE analysis of Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free (A323871) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.

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Figure 2: Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free (A323871)

SEC-HPLC analysis of Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free (A323871) confirms that the purity of this antibody is 99.25%.

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Figure 3: Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free (A323871)

Immobilized recombinant human 5T4 protein (His tag) at 2 µg/ml is bound by Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free (A323871). EC₅₀ = 0.004604 µg/ml.

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Figure 4: Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free (A323871)

Human 5T4 HEK293 cells were stained with Anti-5T4 Antibody [PF-06263507] - Low endotoxin, Azide free (A323871) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC₅₀ = 0.7286 µg/ml.

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