Anti-4E-T Antibody (A88511)

Name: Anti-4E-T Antibody
Brand: Antibodies.com
SKU: A88511
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to 4E-T for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-4E-T Antibody

Description

Rabbit polyclonal antibody to 4E-T.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EIF4ENIF1 (NP_001157973.1).

Sequence

LGGRRIGSGRIISARTFEKDHRLSDKDLRDLRDRDRERDFKDKRFRREFGDSKRVFGERRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

178 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

2610509L04Rik, 4E T, 4ET, 4ET_HUMAN, A930019J01Rik, AA410001, AU021239, Clast 4, Clast4, D11Ertd166e, eIF4E transporter, eIF4ENIF1, Eukaryotic translation initiation factor 4E nuclear import factor 1, Eukaryotic translation initiation factor 4E transporter, FLJ21601, FLJ26551, OTTMUSP00000000698, OTTMUSP00000005193, OTTMUSP00000005194, OTTMUSP00000036075

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-4E-T Antibody (A88511)

Western blot analysis of extracts of various cell lines, using Anti-4E-T Antibody (A88511) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunofluorescence - Anti-4E-T Antibody (A88511)

Immunofluorescence analysis of NIH/3T3 cells using Anti-4E-T Antibody (A88511) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-4E-T Antibody (A88511)

Immunofluorescence analysis of PC-12 cells using Anti-4E-T Antibody (A88511) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-4E-T Antibody (A88511)

Immunofluorescence analysis of U2OS cells using Anti-4E-T Antibody (A88511) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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