Rabbit polyclonal antibody to 4E-BP1 (Phospho-Thr70)
Specificity
The antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70.
Applications
IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine70 (T-K-T(p)-P-P) derived from Human 4E-BP1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, eIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Thr70) antibody #11781 (left)or the same antibody preincubated with blocking peptide (right).
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