Rabbit polyclonal antibody to 4E-BP1 (phospho Thr69).
Specificity
This antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr69.
Applications
WB, IHC, ELISA
Dilutions
IHC: 1:50-1:100, ELISA: 1:1000
Reactivity
Mouse, Rat
Immunogen
Synthetic peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69 (amino acids 35-84).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
12kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Figure 1: Western Blot - Anti-4E-BP1 (phospho Thr69) Antibody (A94241)
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30' using Anti-4E-BP1 (phospho Thr69) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human brain carcinoma using Anti-4E-BP1 (phospho Thr69) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human colon cancer using Anti-4E-BP1 (phospho Thr69) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
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