Rabbit polyclonal antibody to 4E-BP1 (phospho Thr36).
Specificity
This antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr36.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:20000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human 4E-BP1 around the phosphorylation site of Thr36 (amino acids 4-53).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
12kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-4E-BP1 (phospho Thr36) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Figure 2: Western Blot - Anti-4E-BP1 (phospho Thr36) Antibody (A94220)
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 30' using Anti-4E-BP1 (phospho Thr36) Antibody. The left hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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