Rabbit polyclonal antibody to 4E-BP1 (phospho Ser64).
Specificity
This antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Ser64.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human 4E-BP1 around the phosphorylation site of Ser64 (amino acids 30-79).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
12kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Anti-4E-BP1 (phospho Ser64) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Figure 2: Western Blot - Anti-4E-BP1 (phospho Ser64) Antibody (A94221)
Western blot analysis of lysates from Jurkat cells treated with Insulin 0.01U/ml 15' using Anti-4E-BP1 (phospho Ser64) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Publishing research using Anti-4E-BP1 (phospho Ser64) Antibody (A94221)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-4E-BP1 (phospho Ser64) Antibody (A94221)
