Anti-14-3-3 zeta (phospho Ser58) Antibody (A94362)

Name: Anti-14-3-3 zeta (phospho Ser58) Antibody
Brand: Antibodies.com
SKU: A94362
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to 14-3-3 zeta (phospho Ser58) for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-14-3-3 zeta (phospho Ser58) Antibody

Description

Rabbit polyclonal antibody to 14-3-3 zeta (phospho Ser58).

Specificity

This antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Ser58.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58 (amino acids 24-73).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

27kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

14 3 3 delta, 14 3 3 protein zeta/delta, 14 3 3 protein/cytosolic phospholipase A2, 14 3 3 zeta, 14-3-3 protein zeta/delta, 1433Z_HUMAN, Epididymis luminal protein 4, Epididymis secretory protein Li 3, HEL S 3, HEL4, KCIP-1, KCIP1, MGC111427, MGC126532, MGC138156, Phospholipase A2, Protein kinase C inhibitor protein 1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide, YWHAD, YWHAZ

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-14-3-3 zeta (phospho Ser58) Antibody (A94362)

Western blot analysis of lysates from NIH/3T3 cells treated with UV 30' using Anti-14-3-3 zeta (phospho Ser58) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-14-3-3 zeta (phospho Ser58) Antibody (A94362)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-14-3-3 zeta (phospho Ser58) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Immunofluorescence - Anti-14-3-3 zeta (phospho Ser58) Antibody (A94362)

Immunofluorescence analysis of HeLa cells, treated with PMA 125ng/ml 30', using Anti-14-3-3 zeta (phospho Ser58) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.