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Anti-TCR alpha + TCR beta Antibody [R73] - BSA and Azide free (A281639)

$830

Mouse monoclonal [R73] antibody to TCR alpha + TCR beta for IHC-Fr, Flow Cytometry, IP and Functional Assays.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Anti-TCR alpha + TCR beta Antibody [R73] - BSA and Azide free
Description
Mouse monoclonal [R73] antibody to TCR alpha + TCR beta.
Specificity
This antibody recognises a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative. Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes.
Applications
IHC-Fr, Flow Cytometry, IP, Functional Assays
Dilutions
Flow Cytometry: 1:50 - 1:100, IHC-Fr: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 100µl of whole blood or 106 cells in 100µl.
Reactivity
Rat, Monkey, Cynomolgus monkey
Immunogen
Rat T Blasts and Erythrocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
R73
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Rat TCR alpha/beta antibody, clone R73 recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).
Synonyms
T cell receptor beta variable 7-9
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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