Anti-NF-H Antibody [NAP4] (A85339)

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-NF-H Antibody [NAP4] (A85339) at a dilution of 1:10,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-NF-H Antibody [NAP4] (A85339) strongly labels Purkinje cell dendrites and the projections of neuronal cells within the granular layer and the white matter. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed rat, and human tissues.

Human cerebellar cortex fixed in formalin, embedded in paraffin and stained with Anti-NF-H Antibody using the ABC (avidin biotin conjugate) method. The section was counterstained with heamatoxylin-eosin (blue). Anti-NF-H Antibody stains prominent basket cell axons surrounding the large Purkinje neurons. Granule cell layer at bottom of image, molecular layer at top.

Western blot analysis of tissue lysates using Anti-NF-H Antibody, at a dilution of 1:10,000, in green,: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, [Lane 5] mouse spinal cord, [Lane 6] pig spinal cord, [Lane 7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with Anti-NF-H Antibody.

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (Lane 1). The remaining lanes show blots of this material stained with various antibodies including Anti-NF-H Antibody (Lane 2).