Mouse monoclonal [MEM-233] antibody to CD80 (APC).
Specificity
This antibody reacts with an extracellular epitope of CD80, a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages, or activated B lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Extracellular domain of human CD80 fused to human IgG1(Fc).
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-233
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of CD80+ transfected P815 cells (red-filled) from P815 cells (black-dashed) in flow cytometric analysis stained with Anti-CD80 Antibody [MEM-233] (APC) (10 µl reagent per milion cells in 100 µl of cell suspension).
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Proteins predicted to interact with CD80
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.