Anti-CD4 Antibody [GK1.5] - BSA and Azide free (A280518)

Anti-CD4 Antibody [GK1.5] - BSA and Azide free

Rat monoclonal [GK1.5] antibody to CD4.

This antibody recognises mouse CD4, a ~55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on T helper cells, peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+ CD25+), which play a key role in the maintenance of self tolerance. Rat anti Mouse CD4 antibody, clone GK1.5 has been reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used for in vivo and in vitro cell depletion of CD4+ T-cells.

IHC-Fr, WB, Flow Cytometry, ELISA, IP, IF, Functional Assays

Flow Cytometry: 1:10 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Mouse

Murine CD4.

Rat

Monoclonal

GK1.5

IgG2b

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD4 antibody, clone GK1.5 recognizes mouse CD4, a ~55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on T helper cells, peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+ CD25+), which play a key role in the maintenance of self tolerance. Rat anti Mouse CD4 antibody, clone GK1.5 has been reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used for in vivo and in vitro cell depletion of CD4+ T-cells.

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4