Anti-alpha Synuclein Antibody [3C11] (A304959)

Western blot analysis of mouse, rat brain showing detection of 14 kDa Alpha Synuclein protein using Anti-alpha Synuclein Antibody [3C11] (A304959) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse brain cell lysate. Lane 3: rat brain cell lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at room temperature. Color Development: ECL solution (Super Signal West Pico) for 5 minutes in room temperature. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).

Western blot analysis of human brain showing detection of 14 kDa Alpha Synuclein protein using Anti-alpha Synuclein Antibody [3C11] (A304959) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: human brain cell lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at room temperature. Color Development: ECL solution (Super Signal West Pico) for 5 minutes in room temperature. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).

Immunohistochemistry analysis of rat cerebellum, fixed in formalin. The Primary Antibody used was Anti-alpha Synuclein Antibody [3C11] (A304959) at 1:25 for 1 hour at room temperature. The secondary antibody used was Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Alpha Synuclein Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-alpha Synuclein Antibody [3C11] (A304959), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.