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Anti-Akt1 (phospho Thr450) Antibody (A94467)

Western Blot - Anti-Akt1 (phospho Thr450) Antibody (A0406) - Antibodies.com Immunohistochemistry - Anti-Akt1 (phospho Thr450) Antibody (A0406) - Antibodies.com Western Blot - Anti-Akt1 (phospho Thr450) Antibody (A0406) - Antibodies.com Western Blot - Anti-Akt1 (phospho Thr450) Antibody (A0406) - Antibodies.com Immunohistochemistry - Anti-Akt1 (phospho Thr450) Antibody (A0406) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to Akt1 (phospho Thr450) for WB, IHC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-Akt1 (phospho Thr450) Antibody
Description
Rabbit polyclonal antibody to Akt1 (phospho Thr450).
Specificity
This antibody detects endogenous levels of Akt1 only when phosphorylated at Thr450.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Akt1 around the phosphorylation site of Thr450 (amino acids 416-465).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
55kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

Western Blot - Anti-Akt1 (phospho Thr450) Antibody (A0406) - Antibodies.comEnlarge Image

Western Blot - Anti-Akt1 (phospho Thr450) Antibody (A94467)

Western blot analysis of lysates from NIH/3T3 cells treated with PDGF 50ng/ml 20' using Anti-Akt1 (phospho Thr450) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-Akt1 (phospho Thr450) Antibody (A0406) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Akt1 (phospho Thr450) Antibody (A94467)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Anti-Akt1 (phospho Thr450) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Akt1 (phospho Thr450) Antibody (A0406) - Antibodies.comEnlarge Image

Western Blot - Anti-Akt1 (phospho Thr450) Antibody (A94467)

Western blot analysis of various cells using Anti-Akt1 (phospho Thr450) Antibody.
Western Blot - Anti-Akt1 (phospho Thr450) Antibody (A0406) - Antibodies.comEnlarge Image

Western Blot - Anti-Akt1 (phospho Thr450) Antibody (A94467)

Western blot analysis of 453 cells using Anti-Akt1 (phospho Thr450) Antibody.
Publishing research using Anti-Akt1 (phospho Thr450) Antibody (A94467)? Please let us know so that we can list the citation on this page.

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Proteins predicted to interact with AKT1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score