Mouse CXCL9 ELISA Kit (A1559)

Mouse CXCL9 ELISA Kit

Sandwich (quantitative)

Mouse CXCL9 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CXCL9 in the samples. A monoclonal antibody specific for mouse CXCL9 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for CXCL9, and CXCL9 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CXCL9 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

15.63 - 1000 pg/ml

This kit can assay both natural and recombinant mouse CXCL9.

Mouse

This assay has shown no significant cross-reactivity.

98% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

C-X-C motif chemokine 9, CMK, Gamma-interferon-induced monokine, HuMIG, MIG, Monokine induced by interferon-gamma, SCYB9, Small-inducible cytokine B9