Primary Antibodies
Secondary Antibodies
Proteins & Peptides
ELISA Kits
About Us
Contact Us
Antibodies.com Logo Antibodies.com Logo
Sign In/Register
0
ISO 9001:2015 Certified
Live Customer Support
4.5/5 on Trustpilot
100% Quality Guarantee

Anti-Soft-Shelled Turtle Iridovirus Aptamer [QA-36] (Biotin) (A321103)

1 Citation
Secondary Structure - Anti-Soft-Shelled Turtle Iridovirus Aptamer [QA-36] (Biotin) (A321103) - Antibodies.com
$755
Product Datasheet
100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 20-35 business days.

  • Specifications

Name
Anti-Soft-Shelled Turtle Iridovirus Aptamer [QA-36] (Biotin)
Description
Synthetic DNA aptamer to Soft-Shelled Turtle Iridovirus [QA-36] (Biotin).
Specificity
The aptamer only recognized the STIV virus when its binding was tested for other viruses.
Aptamer ID
QA-36
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Binding Buffer: 20mM Tris-HCl Buffer, pH 7.5, with 100mM Sodium Chloride and 2.5mM Magnesium Chloride.
Sequence
5’-dTpdGpdTpdGpdCpdGpdGpdGpdGpdGpdApdGpdGpdGpdGpdApdGpdTpdGpdGpdCpdGpdCpdTpdGpdTpdTpdGpdGpdTpdGpdCpdGpdGpdGpdTpdApdTpdApdGpdCpdGpdCpdGpdTpdGpdGpdTpdGpdTp-3’
Aptamer Length
50 nt
Aptamer Molecular Weight (Da)
15,794.2 Da
Aptamer Affinity
Affinity (Kd) = 53.8 nM
Extinction Coefficient
478800 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Soft-Shelled Turtle Iridovirus Aptamer [QA-36] (Biotin) (A321103) is a replica of the aptamer described in PMID: 26419355.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 20-35 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Soft-Shelled Turtle Iridovirus Aptamer [QA-36] (Biotin) (A321103) - Antibodies.comEnlarge Image

Anti-Soft-Shelled Turtle Iridovirus Aptamer [QA-36] (Biotin) (A321103)

Secondary structure of Anti-Soft-Shelled Turtle Iridovirus Aptamer [QA-36] (Biotin) (A321103).

  • Product Citations (1)

PubMed LogoView Publication
Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects
This publication cites Anti-Soft-Shelled Turtle Iridovirus Aptamer [QA-36] (Biotin) (A321103).
Article Abstract

Background: Soft-shelled turtle iridovirus (STIV) causes severe systemic disease in farmed soft-shelled turtles (Trionyx sinensis). More efficient methods of controlling and detecting STIV infections are urgently needed.

Methods: In this study, we generated eight single-stranded DNA (ssDNA) aptamers against STIV using systematic evolution of ligands by exponential enrichment (SELEX).

Results: The aptamers formed representative stem-loop secondary structures. Electrophoretic mobility shift assays and fluorescent localization showed that the selected aptamers had high binding affinity for STIV. Aptamer QA-36 had the highest calculated binding affinity (K d ) of 53.8 nM. Flow cytometry and fluorescence microscopy of cell-aptamer interactions demonstrated that QA-12 was able to recognize both STIV-infected cells and tissues with a high level of specificity. Moreover, the selected aptamers inhibited STIV infection in vitro and in vivo, with aptamer QA-36 demonstrating the greatest protective effect against STIV and inhibiting STIV infection in a dose-dependent manner.

Discussion: We generated DNA aptamers that bound STIV with a high level of specificity, providing an alternative means for investigating STIV pathogenesis, drug development, and medical therapies for STIV infection.

Conclusions: These DNA aptamers may thus be suitable antiviral candidates for the control of STIV infections.

View Publication
Publishing research using Anti-Soft-Shelled Turtle Iridovirus Aptamer [QA-36] (Biotin) (A321103)? Please let us know so that we can list the citation on this page.